Partial purification of the Escherichia coli K-12 mec+ deoxyribonucleic acid-cytosine methylase: in vitro methylation completely protects bacteriophage lambda deoxyribonucleic acid against cleavage by R-EcoRII
نویسندگان
چکیده
منابع مشابه
The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IX. Deoxyribonucleic acid methylase in bacteriophage-infected Escherichia coli.
Infection of Escherichia coli B with bacteriophages Tl, T2, or T4 results in increase in the activity of deoxyribonucleic acid methylase assayed in crude extracts of the infected cells. The largest increase is observed after infection with T2. phage; T4 and Tl phages lead to smaller increases in that order of decreasing magnitude. After infection with T3, T5, or T6 phages, a decrease in activit...
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Deoxyribonucleic acid (DNA) from bacteriophage T7 has been used to monitor the capacity of gently lysed extracts of Escherichia coli to perform repair resynthesis after ultraviolet (UV) irradiation. Purified DNA damaged by up to 100 J of UV radiation per m2 was treated with an endonuclease from Micrococcus luteus that introduces single-strand breaks in irradiated DNA. This DNA was then used as ...
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During the conjugal transfer of the R64-11 plasmid at 42 C from donor cells thermosensitive for vegetative deoxyribonucleic acid (DNA) synthesis to recipient minicells, the plasmids are conjugally replicated in the donor cells. This conjugal replication is inhibited by nalidixic acid, and the degree of inhibition is comparable to the reduction in the amount of plasmid DNA transferred to the rec...
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The DNA-unwinding protein from Escherichia coli has been purified to homogeneity. It is a single polypeptide of 22,000 daltons; the native molecular weight is 90,000. The effect of the protein on the activity of the three DNA polymerases of E. coli has been studied. The activities of DNA polymerases I and III are significantly reduced, whereas DNA polymerase II activity is enhanced in the prese...
متن کاملChange in methylation of Salmonella bacteriophage P3 deoxyribonucleic acid with host-controlled modification by Escherichia coli.
Modification of bacteriophage P3 by passage through Escherichia coli K was correlated with a 54% decrease in the content of 6-methylaminopurine in the phage deoxyribonucleic acid.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1977
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.129.3.1330-1334.1977